Coronary artery disease (CAD) refers to the progressive development of atherosclerosis, which is a global significant mortality cause, initial diagnosis of CAD is a challenge. Consequently, biomarkers are required for prediction of subclinical atherosclerosis and early treatment. Previously, miR-126 and miR-423-3p were approved to be participants in (CAD) development. Yet, their association with subclinical atherosclerosis is unidentified. Aim: The aim of our study is to investigate the role of plasma miRNA 126 and miRNA 423-3p levels to predict subclinical atherosclerosis and correlate it with exercise ECG test. Methods: The levels of miR-126 and 423-3p were assessed in plasma from 50 healthy control (group I) and 50 subclinical atherosclerotic CAD cases (group II) by quantitative real time –PCR (qRT-PCR). Exercise ECG was done to detect ischemic changes in both groups. Results: There was a significant decrease of miRNA 126 and miR 423-3p levels in group II compared to group I. Likewise, a positive correlation was detected between both miR 126 and miR 423-3p with positive exercise ECG test. ROC curves of both miR 126 and miR-423-3p to detect positive exercise ECG test showed sensitivity and specificity of 92.2% and 93.9%, correspondingly. The combination of both miR-126 and miR-423-3p is considered as hopeful indicators of subclinical atherosclerosis in risky individuals, thus intensifying the management of hazard factors might decrease atherosclerotic progression.