Background: Cisplatin (CIS) is a common antineoplastic drug used to treat various solid cancers. Crocin possesses anti-inflammatory, anti-cancer, antioxidant, and memory-enhancing properties. Aim of study: This study aimed to study the effect of CIS on prepubertal rat testes and protective effect of Crocin on CIS-induced testicular toxicity. Material and Methods: 90 (5-days) old albino Wistar rats were divided into 3main groups (n = 30 each): (control negative group); rats weren’t given any treatment. (Control positive1): received 0.15 ml saline /day intraperitoneal (i.p) only for 5 days before scarification. (Control positive 2): received Crocin at a dose of (200mg/kg) for 4 consecutive days. (CIS treated group): received Cisplatin (CIS) as a single subcutaneous injection in the back of the neck one day just before scarification and subsequent laparotomy at a dose of 5μg/g. (CIS+Crocin group): received200 mg/kg of Crocin for 4consecutive days before treatment with CIS. The last dose of Crocin was given one hour before CIS. Each group was further divided into 3 subgroups based on rat age on the day of sacrifice (PND11, PND17, and PND30). Rats were anesthetized; their testes were extracted and subjected to histological, immunohistochemical, and morphometric examinations. Results: CIS administration significantly decreased body weights, testicular weights, and serum testosterone levels in all age’s groups. Also, it caused degeneration of testicular cells with loss of cellular organization, marked cellular atrophy, and disruption of tubular basement membranes. A significant decrease in tubular diameter and a significant increase in percentage of apoptotic cells were observed in the CIS-treated rats. Crocin administration minimally mitigated these hazardous effects of CIS in the prepubertal rat testes. Conclusion: These findings demonstrated that CIS has a cytotoxic and apoptotic effect on the testicular germinal epithelium and Crocin could minimally improve these alterations.