CombinedserummiR-29candmiR-149 expressionanalysisasdiagnosticgenetic markersforcolorectalcancer

Faculty Medicine Year: 2020
Type of Publication: ZU Hosted Pages:
Authors:
Rasha Shaaban AbdelMaksud
Journal: Biotechnology and Applied Biochemistry Wiley Online Library Volume:
Keywords : CombinedserummiR-29candmiR-149 expressionanalysisasdiagnosticgenetic markersforcolorectalcancer    
Abstract:
CirculatingmiRNAsgatheredmuchinterestincancerresearch asnoninvasivebiomarkers.Theaimofthisstudywasto analyzetheexpressionofmiR-29candmiR-149among colorectalcancer(CRC)patientsandtoexploretheir diagnosticandprognosticpotentialsinrelationtotheclinical andpathologicalfeatures.TheexpressionlevelsofmiR-29c andmiR-149wereevaluatedintheseraof80CRCpatients,80 colorectaladenoma(CRA)patients,and80healthycontrols usingquantitativerealtimepolymerasechainreaction(PCR). Carcinoembryonicantigenserumlevelswereassayedusing enzyme-linkedimmunosorbentassay.miR-29candmiR-149 weresignificantlydownregulatedamongCRCpatients comparedwithCRAandcontrols(miR-29c,0.54±0.19vs. 0.86±0.12,0.99±0.07,P<0.001,respectively;miR-149, 0.46±0.19vs.0.74±0.012,1.0±0.22,P<0.001,respectively). miR-29candmiR-149significantlyassociatedwithadvanced stagesofCRC,tumorsize,andlymphaticmetastasis.Byusing receiveroperatingcharacteristiccurveanalysis,combined miR-29candmiR-149revealedthehighestdiagnosticpotential forCRA(areaunderthecurve[AUC]=0.967)fromhealthy controlsaswellasthediagnosisofCRC(AUC=0.98)from CRA.Moreover,combinedmiRNAsrevealedhighdiagnostic potentialfortheearlierstagesofCRCcomparedwith advancedstages(AUC=0.96).Inconclusion,combinedserum miR-29candmiR-149expressionanalysisestablishednovel noninvasivebiomarkerforearlyCRCdiagnosis. ©2020 InternationalUnionofBiochemistryandMolecularBiology,Inc.Volume68, Number4,Pages732–743,202
    
     
 
       

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