Objective: The carbonated soft drinks (CSDs) consumption has markedly increased worldwide in the last decades, for both the number of servings/given time and the quantity per serving. Variable health problems have been linked to CSDs consumption. This aroused suspicion about a genotoxic effect in addition to the cytotoxicity investigated. This experimental study aimed to evaluate thepossible harmful effects of caffeinated versus non-caffeinated SDs on DNA and cellular integrity. Material andMethods: forty adult Wistar albino rats were allowed for free intake of two groups of CSDs (caffeinated and non-caffeinated) separately for 3months, while observing their bodyweights. After scarification, blood biochemistry, histopathological examination, comet analysis and morphometry were done to detect statistical significance of the findings. Results; caffeinated CSDsproved statistically to have a biochemical, genotoxic, and cytotoxic effects worse than the non-caffeinated CSDs. Conclusion: the deleterious genotoxic and cytotoxic effects of CSDs necessitates precise measures to control their free high consumption levels. Key words: Domestic beverages, genotoxicity, cytotoxicity, comet assay. Corresponding Author: Dr. Enssaf Ahmed AhmedE. mail: eaabdelhamid@medicine.zu.edu.eg