Background: Ankylosing spondylitis (AS) is a chronic inflammatory condition that affects the sacroiliac, and peripheral joints. Systolic dysfunction in AS by conventional assessment may not detect early impairment, so we aimed to study the role two dimensional (2D)-speckle tracking echocardiography (STE) in assessment of the ventricular function and correlate these findings with AS disease activity score (ASDAS). Methods: This study is a case-control study involving 135 participants; they were classified into three groups, inactive group (I) 57 cases, active group (II) 33 cases, and 45 control group (III) . The ASDAS was calculated for all patients. Laboratory parameters ;c–reactive protein (CRP),lipid profile, conventional echocardiographic assessment of ventricular systolic function and (2D) (STE) on both venticles were done Results: ASDAS was significantely high in the the active group (II). Right ventricular free wall strain (RVFWS) was more impaired in the same group p-value <0.001. Pulmonary artery systolic pressure (PASP) was also high in the same group p-value <0.001. AS patients were divided into two subgroups according to drug type biological 70 cases versus non-biological 20 cases. RVFWS was more impaired in the biological drug group -18.35%±3.08 versus 20.76±2.21 in the non-biological group, p-value 0.002. The predictor of impaired RVFWS in AS was PASP (p= 0.001), Exp (B) 1.143, 95% C.I: 6.895 to 13.714. RVFWS had a significant negative correlation with ASDAS-CRP, and PASP