Detection of phylogrouping, adhesin, and extended-spectrum β-lactamase genes in hospital-acquired uropathogenic Escherichia coli isolates

Faculty Medicine Year: 2024
Type of Publication: ZU Hosted Pages:
Authors:
Journal: Molecular Biology Reports Springer Nature Volume:
Keywords : Detection , phylogrouping, adhesin, , extended-spectrum β-lactamase genes    
Abstract:
Background: It has been interesting to compare the levels of antimicrobial resistance and the virulence characteristics of uropathogenic Escherichia coli (UPEC) strains of certain phylogenetic groups. The purpose of this study was to identify the frequency of phylogenetic groups, adhesin genes, antibiotic sensitivity patterns, and extended spectrum beta-lactamase (ESBL) genes in hospital-acquired UPEC. Methods After UPEC isolation, the disc diffusion method was used to assess its susceptibility to antibiotics. Combination disc testing confirmed the existence of ESBL producers. Polymerase chain reaction (PCR) was used to detect genes for adhesin and ESBLs. Results One hundred and twenty-eight E. coli were isolated, which had the highest resistance to tetracycline (96%), followed by cefoxitin (93%), cefepime (92%), ceftazidime (79%), aztreonam (77%), and sulfamethoxazole-trimethoprim (75%). About 57% of isolates were phenotypically ESBL-positive, and they were confirmed by PCR. B2 phylogroup (41%) was the most frequent in E. coli isolates, then group D (30%), group A (18%), and lastly group B1 (11%). ESBL genes were more significantly prevalent in phylogroups B2 and D than other phylogroups (P < 0.001). Regarding adhesin genes, both fimH and afa were more significantly associated with group B2 than other groups (P < 0.009, < 0.032), respectively. In ESBL-positive isolates, both genes were more significantly detected compared to negative ones (P < 0.001). Conclusion Phylogroups B2 and D of UPEC are important reservoirs of antimicrobial resistance and adhesion genes. Detection of ESBL-producing E. coli is important for appropriate treatment as well as for effective infection control in hospitals.
   
     
 
       

Author Related Publications

  • Hana Mohammed Ismail Maghrabi, "Diagnostic performance of direct latex agglutination, post-enrichment latex agglutination and culture methods in screening of group B streptococci in late pregnancy: a comparative study", Dove Medical Press Ltd, 2019 More
  • Hana Mohammed Ismail Maghrabi, "Acr AB and Oxq AB Efflux Pump Genes among Resistant Klebsiella pneumoniae Isolated from Zagazig University Hospitals", Egyptian Society for Medical Microbiology, 2024 More
  • Hana Mohammed Ismail Maghrabi, "Phenotypic and molecular characterization of carbapenem-resistant Acinetobacter baumannii isolates from ventilator associated pneumonia patients.", Zagazig University, 2025 More
  • Hana Mohammed Ismail Maghrabi, "Human leukocyte antigen-B27 and antibodies to Klebsiella pneumoniae in Ankylosing Spondylitis: associations and clinical outcome", Modern commerical press, 2023 More
  • Hana Mohammed Ismail Maghrabi, "Correlation between serum level of interleukin-33 and relative expression of toll like receptor 4 in systemic lupus erythematosus patients", Modern commerical press, 2025 More

Department Related Publications

  • Heba Mohamed Kadry Ahmed Shalapy, "Assessment of serum and urinary levels of neutrophil gelatinase-associated lipocalin in correlation with albuminuria in nondiabetic obese patients تقييم مستويات المصل والمستويات البولية من بروتين الليبوكالين المرتبط بالجيلاتينيز في الارتباط مع البول الزلالي لدى مرضى السمنة غير المصابين بالسكرى", The Egyptian Journal of Internal Medicine, 2019 More
  • Takwa Alsayed Mohamed Meawad, "Efficacies of echinocandins versus azoles antifungal agents against Candida albicans and Candida dubliniensis isolates", The InTernaTIonal arabIc Journal of anTImIcrobIal agenTs Issn 2174-9094 © Under License of Creative Commons Attribution 3.0 License 1, 2014 More
  • Reham Mohamed Elshabrawi Mohamed, "Burden of Fungal Allergic Reactions and Response to Subcutaneous Immunotherapy", Egyptian Journal of Medical Microbiology, 2016 More
  • Maha Kamal Abdel Razek, "Epidemiology of Hepatitis C Virus Infection in Egypt", SCIENCEDOMAIN international, 2015 More
  • Marian Asaad Gerges Sadaq, "Performance Characteristics of Enzyme Linked Immunosorbent Assay and Rapid Immunochromatographic Test for Routine Screening of Human Norovirus", AFRICAN JOURNAL OF CLINICAL AND EXPERIMENTAL MICROBIOLOGY, 2016 More
Tweet