This study was conducted to evaluate the effect of adding multi-strain probiotics (MSP) with or without dry yeast to the diet on blood, immune characteristics and antioxidant status of male Saidi sheep. Five males (54.14 1.67 kg average body weight) were used and randomly distributed in a 55 Latin square design to evaluate MSP administration for a period of 105 days. The five treatments were given the basic diet (50:50% rough/concentrate), where: the first treatment was control and without other additives, while 2nd and 3rd groups were added at a rate of 1 g/day/animal to the other four treatments at a level of (2 x 10 cfu/g) or (4 x 10 cfu/g), ABLB2 and ABLB4, respectively. The 4th (ABLB2+SC) and 5th (ABLB4+SC) treatments were ABLB2 and ABLB4 enriched with 1 g/day/animal of dry yeast Saccharomyces cerevisiae (SCC) was added at a level of (2x 10 cfu/g). The results showed that adding MSP to the basal diet had no effect on blood parameters. While ABLB4 and ABLB4+SC groups had better values of GSH-Px and TAC and significantly reduced the level of MDA compared to the control (P<0.001). There was a slight improvement in the IgM concentration, along with a significant increase in IgA and IgG levels, as well as lysozyme activity in the blood serum in all MSP groups. In conclusion, adding multi-strain probiotics with yeast to the basic diet enhanced the antioxidant capacity and immune response in blood serum in Egyptian male sheep.