Zinc Oxide nanoparticles (ZnO-NPs) are widely used in pharmaceuticals, foods, cosmetics, and toothpaste. The cardiovascular system is one of the most vulnerable exposed tissues to ZnO-NPs. Arabic Gum (AG) is an antioxidant that can be utilized to treat tissue injury. The purpose of this study was to investigate ZnO-NPs-induced cardiotoxicity and the potential protective role of AG. The study included 60 adult male albino rats. They were separated into 3 main groups of 20 rats each (control, ZnO-NPs treated, and protected AG+ ZnO-NPs). Each main group is equally subdivided into 2 subgroups with 10 rats each (control negative with no treatment at all, control positive received AG), ZnO-NPs (100 mg/kg Body weight(BW) and 400mg/kg/BW) and protected (AG + 100 mg/kg ZnO NPs, AG+ 400 mg/kg ZnO-NPs). The dose of AG was 15 mg/kg/BW for tested groups receiving it as was mentioned. The medications were administered orally once a day for four weeks. When comparing the ZnO-NPs treated group versus the control one, there was a substantial rise in mean values of heart enzymes and inflammatory cytokines. Also cardiac histological analysis revealed deteriorated myocardium, vacuolated cytoplasm, and increased fibrosis with apoptosis in ZnO-NPs treated rats. These results were improved by concomitant administration of AG.