Self-healing concrete presents an innovative and eco-friendly solution with the potential to reduce maintenance costs through self-activated crack repair. This study examined the effect of varying bacterial content across different environmental conditions. Additionally, the coupling effect between bacteria and steel fiber to enhance the mechanical and physical properties at different ages has been investigated. Bacillus Sphaericus (B. Sphaericus) was incorporated into the concrete at concentrations of 2 × 109 (CFU/ml), and different bacterial percentages selected utilizing calcium lactate as a nutrient source. Bacterial content of 0.0 %, 0.25 %, 1.0 %, and 2.5 %, cured in both fresh water and magnesium sulfate water solution, were tested to examine concrete behavior in harsh environments. Additionally, steel fibers at volume fraction percentages (Vf%) of 1.0 % and 1.5 %, along with 1.0 % bacterial content …