Background: Precise sex assessment relies over dimorphic dimensions measuring among unknown human remains is a necessary prerequisite for individuals’ identification. Clavicle is a flat short bone appears to be better maintained than long bones. Anthropometric analyses of clavicles to determine the sex are evaluated in different populations with high accuracy. Three-dimensional computed tomography (3D-CT) pictures are an effective technique for this. Objective: The aim of the current study was to study sexual dimorphism of the clavicle, as well as establishment of sex prediction equation among Libyan people by utilizing measurements of the clavicle which obtained from 3D-CT images. Subjects and methods: A total of 50 males and 50 females sexes dimensions were measured from 3D CT scans of the left clavicle: C1, C2 and C3 maximum clavicular length, midshaft circumference, and maximum diameter of the midshaft. C4, C5 and C6 indicated minimum midshaft diameter, maximum sternal end, and acromial end widths, respectively. Results: All the studied measurements in the left clavicle showed a statistical significant increase in males compared to females. C4 was the most single sexually dimorphic dimension of the left clavicle with 90% accuracy. Stepwise discriminant analyses of clavicular variables (C2, C4, and C6) yielded high accuracy at the rate of 99 % (99 % cross-validated). Conclusion: 3D-CT morphomertric analyses of clavicle provide valuable data for identification of sex in modern Libyan population and can be used in forensic investigations.