Background: stroke is considered a major cause of disability worldwide. Vitamin D has been linked to cardiovascular health, including the reduction of stroke risk, while its role in stroke outcome has not been fully studied yet, so we aimed to find the role of vitamin D in the prediction of short-term outcomes of ischemic stroke. Methods: This prospective cohort study was conducted on 58 patients with their first acute ischemic stroke who underwent assessment of their serum vitamin D and their functional outcome three months later by modified Rankin scale. Results: serum vitamin D had a significant role in the prediction of poor short-term outcomes. The mean vitamin D level in our patients was 14.01 ± 8.94ng/ml. Conclusions: the poor short-term outcome of a stroke at three months can be predicted by the presence of hypovitaminosis D, so vitamin D supplementation should be taken into consideration when managing ischemic stroke patients.