According the reduction of edible oil produced in Egypt. It can be used the ultrasound encouragement as a biophysical factor, for seed vitality stimulating. Seeds of soybean and sunflower, were treated by calibrated ultrasound generator, designed and assembled locally, varying two factors, different levels of ultrasonic waves frequency "USF"(20, 30 and 40 kHz) and different exposer time "ET" (0, 10, 20 and 30 min). Seeds were grown till germination ceased, under laboratory conditions to determine their response to USF& ET. Results, were evaluated in terms of the final germination percentage "FGP" (%), germination speed "GRI" (seed day-1), germination energy "GE" (%), and seedling vigor index I (SVI) using split plot design. Results affirmed that ultrasonic treatments have a bio-positive significant effect on measuring indices. The germination period for all treated seeds reduce about 33.34% compared with the control. The USF had direct relations with all parameters, while, the ET has a constrictive effect on all parameters till 20min. Except GE, prolonged ET more than 20 min. reduced all other indices. The highest FGP, GR and GE for soybean and sunflower seeds were (94 and 90%), (50.20 and18.75 seed d−1), and (89.36 and 93.33%) followed 20 min. & 40 kHz. While, the maximum values for SVI (655.20 and 490.20) was obtained at 30min. & 40 kHz. This study confirms that ultrasound applied in agriculture may contribute to the improvement of agro-biological indicators, i.e., FGP, GRI, GE, and SVI for soybean and sunflower seeds.