Background: COVID-19 is an emerging public health problem threatening the life of people globally, especially healthcare providers. Primary health care providers provide the first line of care in communities during health pandemics. Aim of the study was to assess level of concern, compliance, and barriers to use standard precautions among primary health care providers during COVID-19 pandemic. Subjects & Methods: Research design: Descriptive analytical design was utilized to carry out this study. Setting: This study was conducted at all primary health care centers at Al-Mahallah Al-Kubra city, Gharbia Governorate, Egypt. Subjects: A convenient sample of 350 health care providers working in the selected primary health care centers participated in the study. Data collection tool: A structured self-administrated questionnaire consisted of four parts; personal and work data, level of concern, compliance with infection control standard precautions, and barriers to use standard precautions. Results revealed that the majority of study subjects were female nurses, 73.0% and 27% of them had moderate and high level of concern regarding the COVID-19 pandemic respectively. While 54.9% of the studied healthcare providers had high compliance with standard precautions during COVID-19 pandemic. The highly perceived barriers were increased workload (59.1%), lack of sanitizer alcohol for routine hand hygiene (58.9%), and shortage of health care providers (56.9%). A statistically significant negative correlation was found between health care providers’ concern score and their compliance score. Conversely, the concern score had a statistically significant moderate positive correlation with the barrier score. Conclusion: This study concluded that there was high level of concern among health care providers which subsequently could lead to suboptimal health care service and less effective compliance to infection control measures during the COVID-19 pandemic. Recommendations: Effective approaches should be followed to enhance protection of healthcare providers and minimize their concerns during the pandemic as well as primary health care centers pandemic preparedness is needed.