Abstract: A. paniculata are spread throughout south India and Srilanka which perhaps represent the centre of origin and diversity of the species. A. paniculata is of common occurrence in most of the places in our country including the plains and hilly areas. It is one of the important ingredients in Ayurvedic preparations recommended for fever and liver diseases. Andrographolide, chief constituent extracted from the leaves of the plant. The hepatoprotective action of andrographolide is related to activity of certain metabolic enzymes. Andrographis is the herb of Andrographis paniculata, a flowering plant in the Acanthaceae family. The active constituents of Andrographis paniculata are saponins, alkaloids, and phytosterols. 1-β-arabinofuranosylcytosine (Cytosine arabinoside) (Ara-C) is the most important antimetabolite chemotherapeutic drug used for acute leukemia. In this study we examined the protective property of an ethanolic extract of Andrographolide paniculata on biochemical changes in chick embryo. CA caused biochemical changes in a concentration and time dependent manner in amniotic fluid, liver and heart tissues