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Methotrexate enhances oxidative stress, apoptosis, and ultrastructural alterations in the placenta of rat الميثوتريكسات يعزز الإجهاد التأكسدي ، وموت الخلايا المبرمج ، والتغيرات التركيبيه الدقيقه في مشيمة الجرذ
Faculty
Medicine
Year:
2022
Type of Publication:
ZU Hosted
Pages:
Authors:
Shirin Elsayed Tawfiq Othman
Staff Zu Site
Abstract In Staff Site
Journal:
Ultrastructural Pathology Ultrastructural Pathology
Volume:
Keywords :
Methotrexate enhances oxidative stress, apoptosis, , ultrastructural
Abstract:
The chemotherapeutic drug methotrexate (MTX) is utilized to treat various malignancies. MTX exposure during pregnancy causes miscarriages, abnormalities in newborns, and developmental delays. The current study examined the placenta’s sequential histopathological alterations follow ing exposure to the MTX in pregnant rats. Twenty-four pregnant rats were assigned into; the control group and MTX group (0.2 mg/kg). MTX was given intraperitoneally on gestational days 11–12. Oxidative stress parameters were measured in placental homogenates. The placental specimens were evaluated by light, immunohistochemical (caspase-3 and vascular endothelial growth factor (VEGF)), and electron microscopic study. Malondialdehyde levels were significantly elevated by MTX, whereas glutathione peroxidase and superoxide dismutase levels were significantly reduced. The MTX group showed a marked reduction in the thickness of both the basal and labyrinth zones. Degeneration of the labyrinth zone was demonstrated. Also, giant trophoblast cells and the spongiotrophoblasts of the basal zone showed vacuolations with dark nuclei. Up-regulation of caspase-3 and down-regulation of VEGF immunoexpression were demonstrated. Ultrastructurally, disintegration of the interhemal membrane, spongiotrophoblasts with vacuolated cytoplasm and small condensed nuclei, and the giant trophoblasts with irregular nuclear outlines and vacuolated cytoplasm were demonstrated. In conclusion, MTX has profoundly altered the structure of the placenta.
Author Related Publications
Shirin Elsayed Tawfiq Othman, "EVALUATION OF TIME-DEPENDENT CHANGES OF THE URINARY BLADDER IN INDUCED DIABETES MELLITUS IN ADULT ALBINO RATS", Ain Shams Medical Journal, 2015
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Shirin Elsayed Tawfiq Othman, "Evaluation of the therapeutic role of stem cells in experimental acetaminophen induced hepatotoxicity in adult male albino rat تقييم الدور العلاجي للخلايا الجذعية في السمية الكبدية التجريبية الناجمة عن عقار الاسيتامينوفين في ذكر الجرذ الابيض البالغ", European journal of Anatomy (Eur J Anat), 2021
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Shirin Elsayed Tawfiq Othman, "Evaluation of Dose Related Structural Changes in Sodium Valproate-Induced Hepatotoxicity and a Possible Protective Role of Vitamin E in Adult Albino Rats تقييم للتغيرات التركيبية المتعلقة بجرعة فالبروات الصوديوم في سمية الكبد والدور الوقائي الممكن لفيتامين ه في الفئران البيضاء البالغة", Egyptian journal of histology, 2020
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Shirin Elsayed Tawfiq Othman, "The neuroprotective effect of ginsenoside Rb1 on the cerebral cortex changes induced by aluminium chloride in a mouse model of Alzheimer’s disease: A histological, immunohistochemical, and biochemical study التأثير الوقائي العصبي للجينسنوسيد Rb1 على تغيرات القشرة الدماغية التي يسببها كلوريد الألومنيوم في نموذج لمرض الزهايمر في الفأر: دراسة نسيجية وكيميائية مناعية وكيميائية حيوية", Journal of Chemical Neuroanatomy, 2023
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Shirin Elsayed Tawfiq Othman, "Lemongrass Essential Oil Attenuates Perfluorooctane Sulfonate-Induced Jejunal Mucosal Injury in Rat: A Histological, Immunohistochemical, and Biochemical Study زيت عشبة الليمون العطري يخفف من إصابة الغشاء المخاطي للصائم التي يسببها سلفونات فلورو أوكتان المشبع في الجرذ: دراسة نسيجية وكيميائية مناعية وكيميائية حيوية", Microscopy and Microanalysis, 2023
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Abdelmonem Awad Mostafa Hegazy, "Anatomy is a misnomer to aims of its study", Academia Anatomica International; Society for Health Care & Research Development; India, 2017
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