Background: Gentamicin is a powerful aminoglycoside antibiotic which is widely used in the proper eradication of gram negative organisms. Acute renal failure is a common complication that develops rapidly through a dose-dependent mechanism after gentamicin administration. Nebivolol is a third generation vasodilator selective β-1 adrenergic receptor blocker which has a potent antioxidant properties that that could ameliorate the renal adverse effects which could be exerted by gentamicin administration. The Aim of work: Is to elucidate the protective effect of nebivolol in gentamicin induced nephrotoxicity. Material and Method: Thirty adult male albino rats weighing 180-220 gm were used in this study. The rats were divided into three groups( 10 rats each for each group): Group I (control group). Group II (Gentamicin treated group). Group III (Gentamicin and nebivolol treated group).The total duration of the experiment was 30 days (one month). Rats of all groups were anaesthetized then the rats were sacrified at day 31(the day after the end of experiment). Results: The administration of gentamicin resulted in shrinkage of the glomeruli, there were widened urinary space and also most of proximal and distal convoluted tubules presented pyknotic and karyolitic nuclei, there was extensive cytoplasmic vacuolation,there were a significant elevation of blood urea nitrogen ,a significant elevation of creatinine levels and metalloproteinase matrix 9 (MMP 9) which were improved markedly by administration of nebivolol drug. Conclusion: It could be concluded that nebivolol may improve the adverse histological and functional changes induced by gentamicin in the adult male albino rat's kidney