Abstract: Norovirus is recognized as a significant cause of gastroenteritis and is a major contributor to non-bacterial gastroenteritis in both industrialized and developing nations. In addition, after rotavirus, Norovirus is thought to be the second most common cause of severe pediatric gastroenteritis. Objective: To determine the effect of educational toolkit on pediatric nurses' knowledge and practices (KP) toward Noroviruses (NoV)among children. Method: 51 nurses working in the pediatrics departments at the Children's Hospital of Mansoura University (MUCH) in El-Mansoura Governorate, Egypt, and they were caring for children with acute gastroenteritis and were willing to participate in the study underwent a five-month quasi-experimental research design (pre-post intervention study). The study was conducted from the first of September2021 to the end of January 2022. Results: The post-implementation scores are significantly higher than the pre- implementation ones. 74.5 vs. 15.7for good sufficient knowledge, and 74.5% vs. 7.8 forgood satisfactory practice. The mean score of nurses' practice about Norovirus and its preventive measures was 27.48 ± 5.89. This mean improved to 41.37 ± 5.70 after the program implementation, with statistically significant differences at p < 0.001. Conclusion: It was concluded that, the educational toolkit had a positive effect on nurses’ knowledge & practices as represented by a significant improvement in the post mean scores of knowledge and practice for nurses at the pediatric medical unit. Recommendations: Integrating Norovirus prevention measures and management into the routine nursing care and creating ongoing educational programs for nurses in response to their requirements/needs aiming to updating their knowledge and enhancing their care of pediatric patients who are infected with such viruses.