ABSTRACT Background: Urothelial carcinoma relapse occurs in the majority of high staged tumors with no improvement in the survival of patients receiving therapy. Mechanisms of urothelial carcinoma should be widely searched to determine the molecular factors and signals responsible for cancer progression, relapse and treatment resistance. Aim: the aim of this work is to evaluate the immunohistochemical expression of FOXO1A and p63 in urothelial carcinoma and investigate their correlation with the prognosis. Methods: Seventy formalin fixed-paraffin blocks of patients of urothelial carcinoma were analyzed for FOXO1A and p63 expressions by immunohistochemistry. Results: FOXO1A was mainly located in the cytoplasm and nucleus of urothelial carcinoma, whereas p63 was detected in the nucleus. There was a highly positive significant correlation between FOXO1A expression and p63 expression in urothelial carcinoma (r 0.32). However, no significant correlation was found between the patients’ age or sex and the expression of FOXO1A or p63. There was a significant increase in the expression of FOXO1A and p63 among low grade carcinomas compared to high grade tumors (p0.02, 0.01, respectively). There was a highly significant increase in the expression of FOXO1A and p63 among tumor stage pTa of the studied cases of urothelial carcinoma compared with higher tumor stages (p<0.001 for both). Conclusions: FOXO1A and p63 are useful prognostic markers for urothelial carcinoma. Overexpression of FOXO1A and p63 in urothelial carcinoma predict better prognosis.