Nabaq (Zizyphus spina-christi L) fruit has a rich composition of bioactive components such as phenolic, flavonoids, dietary fiber, minerals and vitamins. The study was carried out to evaluate the effect of supplementation dried Nabaq fruit on the physicochemical, rheological and sensory properties of low fat yoghurt (1% fat). Dried Nabaq fruit was added to low fat milk yoghurt at ratios of 2 and 4 %.Results showed that gradual increase in pH, dietary fiber, viscosity, phenolic content and antioxidant activity values of low fat yoghurt as dried Nabaq fruit ratio was increased. On the other hand, the acidity and synersis were decreased as the ratio of dried Nabaq fruit was increased. Low fat yoghurt that containing of 4% of dried. Nabaq fruit were the highest of physicochemical, acceptability sensory and rheological properties than the other treatments. Low fat yoghurt fortified with 4% dried Nabaq fruit were evaluated as hypoglycaemic agent streptozotocin-induced diabetic rats. Forty male rats were divided into 5 groups as follows: Group (1) non-treated non-diabetic rats (negative control). Group (2) diabetic rats (received Streptozotocin (STZ), 60 mg/Kg BW) (positive control).Group (3) diabetic rats treated with 20% low fat yoghurt without any additives. Group (4) diabetic rats treated with 20% low fat yoghurt fortified with 4% dried Nabaq fruit. Group (5) diabetic rats treated with Meglitinides.The treatment of diabetic rats with low fat yoghurt containing dried Nabaq fruit showed a significant decreases (p<0.001) in levels of blood glucose, malondialdehyde (MDA), low density lipoprotein (LDL), cholesterol (CL), Triglyceride (TG), AST, ALT, ALP, Creatinin, Uric acid and Urea and increased (p<0.001) Insulin, high density lipoprotein (HDL) and total antioxidant capacity (TAC) in comparison to diabetic control rats. Thus, the study demonstrates that dried Nabaq fruit can be used as a source of phenolic compounds and dietary fiber in low fat yoghurt which enhanced its nutritional value, rheological and sensory properties and possess a hypoglycemia effect.