Abstract: Lemongrass (LGP) is used in folk medicine, and its decoction and infusion are taken as a diuretic. This study aimed to investigate the effects of toast bread supplemented with lemongrass powder on kidney and liver functions in rats. The toast bread recipes were prepared from wheat flour, 5 and 10 % replacement levels of wheat flour (WF) by LGP. The physicochemical and organoleptic properties of the toasted bread were estimated. Thirty-two Male Sprague Dawley rats were used in the study and randomly divided into four groups. Each group consists of eight rats; the 1st group is control group (group A) and fed on a basal diet; 2nd group is GM (group B) fed on basal diet and injected with gentamicin (GM) in a dose of 100 mg/kg b.wt (positive control); the 3rd group injected with GM and fed on a diet supplemented with 20 % WF toast bread containing 10% LGP (group C); the 4th group injected with GM and fed on a diet supplemented with 20 % WF toast bread containing 5% LGP (group D). The results showed that incorporating toast bread fortified with Lemongrass (5% and 10%) induced significant (P<0.05) decreases in serum urea nitrogen, creatinine, ALT, AST, ALP. It also showed hypolipidemic properties and reduced serum cholesterol and triglycerides. These results prove that using toast bread containing Lemongrass might be beneficial for improving vital biomarkers in patient with kidney diseases. Keywords: physicochemical, sensory properties, gentamycin, kidney diseases, lipid Peroxidation