Abstract: The present study aims to investigate the occurrence of aflatoxin B1, the most important mycotoxins and potent natural carcinogen known, in wheat grains samples stored in Egyptian homes. Wheat (Triticum vulgare L.) grains samples were collected from different villages in two Egyptian Governorates, Sharqia and Minia, during the period (2017-2018) and used immediately for Aflatoxin B1 (AFB1), moisture and fat determination. AFB1 in samples were varied from 0.89 to 3.12 and 1.01 to 3.79 μg.kg-1 in Sharqia and Minia governorates samples, respectively. Also, the moisture and fat contents were varied from 12.67 to 16.02 and 1.28 to 1.93 g.100g-1 (in Sharqia) and 12.79 to 15.79 and 1.42 to 1.94 g.100g-1 (In Minia) Governorates samples, respectively. Samples with the higher AFB1 concentration are the samples of higher moisture and fat content. A percent of 33.33 and 41.66% of the tested wheat grains samples recorded AFB1 concentration more than the maximum permissible limits for human consumption (2 μg/kg AFB1) in Sharqia and Minia Governorates, respectively. When all wheat samples were included in the statistical analysis, there was a positive significant (p≤ 0.05) relationship between moisture content (r2 = 0.6878), fat content (r2 = 0.6373) and AFB1 concentration. These correlations confirm that moisture content is mainly participate for the AFB1 concentration of the tested wheat grain samples while fat content are partially participated. Significant variations in AFB1 were observed amongst the samples tested which clearly indicated seasonality and regional factors affected. In conclusion, consumption of some wheat sampled storage by traditional methods can pose a potential risk of development of various diseases in human as the result of such wheat grains with high levels of AFB1. Also, for the proper storage of wheat grains, environmental factors such as moisture content and temperature must be controlled. Keywords: Wheat grains, AFB1, moisture, fat, Minia Governorate, Sharqia Governorate, correlation analysis