Herbicides are widely used for weeds control and potential hazards may have negative effects on the environment and human health. The purpose of this study was to determine quantitatively phytotoxicity during the application of the herbicides. Photosynthetic pigments, total protein contents and its separation electrophoretically, plant defensive enzymes and non- enzymes substances in treated Giza 6 (G6) of peanut leaves (Arachis hypogaea L.) plants at different concentrations of the tested herbicides. The obtained results showed significant differences in chl a chl. b and carotenoids because of the treatments of G6 with the tested herbicides. There were side effects on these photosynthetic pigments concentrations, especially when using the double recommended rate of these herbicides. Protein contents and its separation electrophoretically, the results indicated the absence of some protein bands in leaves treated with the double recommended rate. Respecting the plant defensive enzymes, ascorbate peroxidases (APX), peroxidases (PODs), Superoxide dismutase (SOD) and catalase (CAT), it was found that increased significantly in these defensive enzymes when treated G6 peanut leaves with the double recommended rate. Also, it was found that there was a significant increase in non- enzymes substances (H2O2, MDA and total phenols) in treated G6 peanut leaves with the double recommended rate. So, this study was recommended that avoid using the double recommended rates of these tested herbicides on peanut leaves because of the interactions between these herbicides and some biological processes in the peanut plants and finally reduced the quantitative and qualitative of the product.