Effect of Letrozole on hippocampal Let-7 microRNAs and their correlation with working memory and phosphorylated Tau protein in an Alzheimer's disease-like rat model

Faculty Medicine Year: 2022
Type of Publication: ZU Hosted Pages:
Authors:
Journal: The Egyptian Journal of Neurology, Psychiatry and Neurosurgery SpringerOpen Volume:
Keywords : Effect , Letrozole , hippocampal Let-7 microRNAs , their    
Abstract:
Abstract Background Let-7 microRNAs (miRNAs) may contribute to neurodegeneration, including Alzheimer's disease (AD), but, they were not investigated in Streptozotocin (STZ)-induced AD. Letrozole increases the expression of Let-7 in cell lines, with conflicting evidence regarding its effects on memory. This study examined Let-7 miRNAs in STZ-induced AD, their correlation with memory and hyperphosphorylated Tau (p-Tau) and the effects of Letrozole on them. Methods Seven groups of adult Sprague Dawley rats were used: Negative control, Letrozole, Letrozole Vehicle, STZ (with AD induced by intracerebroventricular injection of STZ in artificial cerebrospinal fluid (aCSF)), CSF Control, STZ + Letrozole (STZ-L), and CSF + Letrozole Vehicle. Alternation percentage in T-maze was used as a measure of working memory. Let-7a, b and e and p-Tau levels in the hippocampus were estimated using quantitative real-time reverse transcription–polymerase chain reaction (qRT–PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Results Significant decreases in alternation percentage and increase in p-Tau concentration were found in the STZ, Letrozole and STZ-L groups. Expression levels of all studied microRNAs were significantly elevated in the Letrozole and the STZ-L groups, with no difference between the two, suggesting that this elevation might be linked to Letrozole administration. Negative correlations were found between alternation percentage and the levels of all studied microRNAs, while positive ones were found between p-Tau concentration and the levels of studied microRNAs. Conclusions This study shows changes in the expression of Let-7a, b and e miRNAs in association with Letrozole administration, and correlations between the expression of the studied Let-7 miRNAs and both the status of working memory and the hippocampal p-Tau levels. These findings might support the theory suggesting that Letrozole aggravates pre-existing lesions. They also add to the possibility of Let-7’s neurotoxicity.
   
     
 
       

Author Related Publications

    Department Related Publications

    • Suzan Mohammed Mohammed Moursi, "Effect of ghrelin on testicular functions in streptozotocin induced type 1 diabetic rats", Corresponding author: miar2009@gmail.com (Dalia I. Abd-Alaleem) Published online at http://journal.sapub.org/diabetes Copyright © 2013 Scientific & Ac, 2013 More
    • Sherif Wageh Mansour, "Formulation and evaluation of metformin hydrochloride-loaded niosomes as controlled release drug delivery system", Drug Delivery, 2013 More
    • Shereen Alarabi Bedeer, "Effects of  –Tocopherol and L-NAME on Experimentally Induced Chronic Hepatic Iron Overload", Sc.J.Az. Med. Fac. (Girls) ., 2013 More
    • Dalia Ibrahim Abdelaleem, "Serum resistin, vaspin and chemerin in rats with non alcoholic fatty liver disease: Correlation with metabolic and haemostatic parameters", Scientific & Academic Publishing, 2014 More
    • Azza Abdelrahman Youssef Megahed, "Serum lipocalin-2 levels in experimentally- induced obese rat with polycystic ovary: Correlation with metabolic and hemostatic parameters", فى مجلة طب الأزهر (بنين), 2016 More
    Tweet