Abstract: Oxytocin is a serious medication that needs close monitoring to avoid consequences on the mother and the fetus and to evaluate labor progress. Aim of the study was to evaluate the effect of educational program on improving nursing knowledge and practice regarding administration of oxytocin during labor. Subjects& methods: A quasi-experimental design was used. The study was conducted in maternity hospital, labor ward, at Zagazig university hospitals. Between the period from the start of October 2018 to the end of March 2019. The study included two groups; Group I: (60 Parturient women; 30 for pre-test & 30 for post- test to evaluate nurses practice regarding oxytocin administration) and Group II: 30 nurses provided direct nursing care for women during labor. Tools: Four tools were used in this study. The first tool was parturient women assessment sheet during labor, the second tool was knowledge questionnaire sheet, the third tool was an observational checklist for nurses' practice, while the fourth tool was intervention program. Results: There were improvement in the total knowledge and practice in post-test intervention than pre-test, with an extremely statistically significant difference (p<0.0001). All nurses had poor knowledge and practice level pre intervention ( 0.0% & 0.0%) which improved to average level post intervention (63.3% &73.3% respectively). Conclusion: There was statistically significant positive correlation between nurse's knowledge and practice score after implementation of the program. Recommendations: Provide in-service training program or workshops to provide the nurse midwives with knowledge and practice about oxytocin drug administration during labor.