Copper (Cu), an essential trace element required for many biological processes inside the body, may cause deleterious effects on several body organs when its administration exceeds the tolerable upper intake level. Recently, great attention has been given to the use of natural compounds that are rich sources of biologically active molecules to prevent and treat many diseases. Therefore, this study was designed to explore the possible protective effects of cinnamon extract and probiotic against nephrotoxicity caused by overdose of Cu in broiler chickens. The whole experiment lasted 6 weeks. Cinnamon extract and probiotic showed remarkable antioxidant, anti-inflammatory, and antiapoptotic properties against the toxic effects of excess Cu in renal tissues of chickens. Based on our results, we conclude that cinnamon extract and/or probiotic can serve as an effective therapeutic option to decrease the renal injury caused by Cu poisoning in broiler chickens