Sixty three does at 6 months of age and twenty seven bucks at 7 months of age of New Zealand White (NZW) were used in an experiment of 3 × 3 factorial design during the hot period of the year (from May to September). Does and bucks were divided randomly into 3 equal groups (21 does and 9 bucks). The groups were allotted to one of the following 3 experimental photoperiods: 12L: 12D (12 hrs lighting and 12 dark), 14L: 10D (14 hrs lighting and 10 dark) and 16L: 8D (16 hrs lighting and 8 dark). Within each photoperiod, does and bucks were randomly distributed to 3 groups of mating times or semen collection times (8 a.m., 2 p.m. and 8 p.m.) and there were 7 does and 3 bucks per each mating time or semen collection time. The results of the present work can be summarized as follows: Doe and their litter traits: Insignificant differences were observed in the physiological body reactions due to the effect of photoperiods. All of doe and litter productive traits studied were significantly (P≤0.01) improved with the short photoperiod (12L: 12D). The lowest (P≤0.05) value of respiration rate was observed in does mated at 8 p.m. AST and ALT were significantly (P≤0.05) differed due to mating time. Rectum temperature, plasma blood total protein, albumin and globulin were insignificantly affected by mating time. Doe and litter productive traits studied were significantly (P≤0.05 and 0.01) higher in does mated at 8 a.m. Insignificant differences were observed in all traits studied of doe due to the effect of the interaction between photoperiod and mating time. Buck traits: Each of the physiological body reactions and reaction time of bucks did not significantly affect by photoperiods or semen collection time. Most of semen characteristics studied was significantly (P≤0.01) improved with short photoperiod (12L: 12D), while dead spermatozoa (%) was significantly (P≤0.05) declined with increasing photoperiod to 16 hrs/ day. Reaction time and semen characteristics studied were better (P≤0.05 and 0.01) when semen collected at 8 a.m. The interaction between photoperiod and semen collection time had insignificant effects on all of buck traits studied.