Possible mechanisms for the renoprotective action of adipose-derived mesenchymal stem cells with CD44 targeted hyaluronic acid against renal ischemia

Faculty Science Year: 2021
Type of Publication: ZU Hosted Pages:
Authors:
Journal: Life Sciences ElSEVIER Volume:
Keywords : Possible mechanisms , , renoprotective action , adipose-derived mesenchymal    
Abstract:
The present study aimed to investigate the invitro preconditioning of adipose-derived mesenchymal stem cells (ADMSCs) with CD44-targeted hyalournic acid (HA) on ischemic kidney injury in rats. Ninety male Sprague Dawley rats were randomly allocated into the following groups; i) sham group, ii) control group: rats exposed to 45 min left renal ischemia with saline treatment, iii) HA group as control group but rats treated with HA, iv) ADMSCs group as control but rats treated with ADMSCs v) HA + ADMSCs group as ADMSCs but rats treated with ADMSCs preconditioned with CD44-tageted HA for 14 days. We found that treattment with either ADMSCs or HA + ADMSCs caused significant decrease in the elevated serum creatinine and BUN and malondialdehyde (MDA) concentrations and expression of TGF-β1, fibronectin, collagen type I, inducible nitric oxide synthease (iNOS) and microRNAs (miR-21, miR-17-5p, miR-10a) in kidney and significant increase in creatinine clearance, superoxide dismutase (SOD), reduced glutathione (GSH) and the expression of Bcl2, vascular endothelial growth factor (VEGF), Wnt/β-catenin pathway genes in kidney compared to control group (p < 0.05). Moreover, HA + ADMSCs group caused more significant improvement in these parameters than ADMSCs group (p < 0.05), while HA group did not cause any significant improvement in these parameters compared to control group. These results suggest that preconditioning of ADMSCs preconditioned with CD44-targted HA enhanced their cytoprotective effect against ischemic kidney injury. This renoprotective effect might be due to activation of angiogenesis, Wnt/β catenin pathway proteins, and suppression of oxidative stress, apoptosis, inflammation and fibrosis.
   
     
 
       

Author Related Publications

  • Mohamed Ali Ibrahim Mohamed ally, "Hepatocyte derived from Rat Bone Marrow Mesenchymal Stem Cells", indian journal of applied research, 2013 More
  • Mohamed Ali Ibrahim Mohamed ally, "Preparation, spectroscopic, characterizations and biological studies of new charge transfer complexes formed between fluconazole drug with various acceptors", ELSEVIER, 2021 More
  • Mohamed Ali Ibrahim Mohamed ally, "Preparation, spectroscopic characterizations and biological studies of a new charge transfer complex formed between fluconazole drug with various acceptors", Elsevier B.V., 2021 More
  • Mohamed Ali Ibrahim Mohamed ally, "Improvement of Renal Failure Using Wharton’s Jelly Derived Mesenchymal Stem Cells", SCIENDO, 2018 More
  • Mohamed Ali Ibrahim Mohamed ally, "Electrospun nanostructured heparin conjugated-poly-ε-caprolactone based scaffold promote differentiation of smooth muscle cells from adipose mesenchymal stem cells", ELSEVIER, 2024 More

Department Related Publications

  • Faten Zahran Mohamed Ibrahim , "Biological studies of the effect of some new synthetic triazole derivatives on ehrlich ascites carcinoma cells. .", International Journal of Biological & Pharmaceutical Research, 2013 More
  • Faten Zahran Mohamed Ibrahim , "Oxidative Stress and Antioxidant Defense in Egyptian Favism Patients", European Review for Medical and Pharmacological Sciences, 2013 More
  • Faten Zahran Mohamed Ibrahim , "Hepatocyte derived from Rat Bone Marrow Mesenchymal Stem Cells", the gloubaljournal, 2013 More
  • Faten Zahran Mohamed Ibrahim , "Expressions of IL10, CXCR3+, CD4+ and CD8+ T cells in Lupus nephritis patients: Correlations with histological classes, activity index and chronicity index", International Journal of Biotechnology and Biochemistry, 2014 More
  • Akabir Tarik Heussein Hassanein, "Mode of Action of Potassium Salt of 2-Thioxo-4-Hydroxycoumarin [3, 4-b] Pyrimidine and 9-Bromo-2-Thioxo-Hydroxycoumarin [3, 4-b] Pyrimidine Against Ehrlich Ascites Carcinoma Cells", Biological and Chemical Research, 2014 More
Tweet