Biochemical and Functional Characterization of Kidney Bean Protein Alcalase-Hydrolysates and Their Preservative Action on Stored Chicken Meat

Faculty Agriculture Year: 2021
Type of Publication: ZU Hosted Pages:
Authors:
Journal: Molecules MDPI Volume:
Keywords : Biochemical , Functional Characterization , Kidney Bean Protein Alcalase-Hydrolysates    
Abstract:
A new preservation approach is presented in this article to prolong the lifetime of raw chicken meat and enhance its quality at 4 °C via coating with highly soluble kidney bean protein hydrolysate. The hydrolysates of the black, red, and white kidney protein (BKH, RKH, and WKH) were obtained after 30 min enzymatic hydrolysis with Alcalase (E/S ratio of 1:100, hydrolysis degree 25-29%). The different phaseolin subunits (8S) appeared in SDS-PAGE in 35-45 kD molecular weight range while vicilin appeared in the molecular weight range of 55-75 kD. The kidney bean protein hydrolysates have considerable antioxidant activity as evidenced by the DPPH-scavenging activity and β-carotine-linolenic assay, as well as antimicrobial activity evaluated by disc diffusion assay. BKH followed by RKH (800 µg/mL) significantly (p ≤ 0.05) scavenged 95, 91% of DPPH and inhibited 82-88% of linoleic oxidation. The three studied hydrolysates significantly inhibited the growth of bacteria, yeast, and fungi, where BKH was the most performing. Kidney bean protein hydrolysates could shield the chicken meat because of their amphoteric nature and many functional properties (water and oil-absorbing capacity and foaming stability). The quality of chicken meat was assessed by tracing the fluctuations in the chemical parameters (pH, met-myoglobin, lipid oxidation, and TVBN), bacterial load (total bacterial count, and psychrophilic count), color parameters and sensorial traits during cold preservation (4 °C). The hydrolysates (800 µg/g) significantly p ≤ 0.05 reduced the increment in meat pH and TVBN values, inhibited 59-70% of lipid oxidation as compared to control during 30 days of cold storage via eliminating 50% of bacterial load and maintained secured storage for 30 days. RKH and WKH significantly (p ≤ 0.05) enhanced L*, a* values, thus augmented the meat whiteness and redness, while, BKH increased b* values, declining all color parameters during meat storage. RKH and WKH (800 µg/g) (p ≤ 0.05) maintained 50-71% and 69-75% of meat color and odor, respectively, increased the meat juiciness after 30 days of cold storage. BKH, RKH and WKH can be safely incorporated into novel foods.
   
     
 
       

Author Related Publications

  • Ahmed Mohamed Saad Mousttafa, "Polyphenolic extracts from pomegranate and watermelon wastes as substrate to fabricate sustainable Silver nanoparticles with larvicidal effect against Spodoptera littoralis", دولي, 2021 More
  • Ahmed Mohamed Saad Mousttafa, "Impacts of Supplementing Broiler Diets with Biological Curcumin, Zinc Nanoparticles and Bacillus licheniformis on Growth, Carcass Traits, Blood Indices, Meat Quality and Cecal Microbial Load", MDPI, 2021 More
  • Ahmed Mohamed Saad Mousttafa, "Green nanotechnology for preserving and enriching yogurt with biologically available iron (II)", Elsevier, 2021 More
  • Ahmed Mohamed Saad Mousttafa, "Selenium nanoparticles from Lactobacillus paracasei HM1 capable of antagonizing animal pathogenic fungi as a new source from human breast milk", Elsevier, 2021 More
  • Ahmed Mohamed Saad Mousttafa, "Phytogenic compounds from avocado (Persea americana L.) extracts; antioxidant activity, amylase inhibitory activity, therapeutic potential of type 2 diabetes", elsevier, 2021 More

Department Related Publications

  • Elsayed Mohamed Abdel Wahed Khalil, ""Detection of Staphylococcus aureus enterotoxins in milk, milk products as well as milk handlers in Sharkia Governorate". الكشف عن السموم المعوية لميكروب ستافيلوكوكس أوريوس في الألبان ومنتجاتها والقائمين بتداولها في محافظة الشرقية", Annals of Agric. Sci., Moshtohor, 2012 More
  • Dalia Ahmed Mohamed Zaki, "" الخواص الكيميائية والريولوجية والحسية للخبز الفينو المدعم بألياف لب المانجو وتأثيره على الفئران المصابة بمرض البول السكرى", كلية التربيه النوعيه - جامعة عين شمس, 2018 More
  • Eman Talaat Ali Mohamed Abosead Ahmed, "Effects of dried okra fruit (Abelmoschus esculentus L.) powder on growth, carcass characteristics, blood indices, and meat quality of stored broiler meat", Elsevier, 2020 More
  • Sameh Abdrabou Elsayed Husseini Korma, "Superheated Steam Pretreatment as a Promising Technique to Improve the Physicochemical Properties of Corn Germ Oil", Wiley, 2025 More
Tweet