Methicillin resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial infections because of its high resistance. Here we study the antibiotic resistance in MRSA clinical isolates and its relation to integron I occurrence. A total of 88 clinical Staphylococcus aureus isolates were collected. MRSA were identified by disk diffusion method (DDM) and confirmed by PCR, antibiogram was determined by DDM. Integron I, II and aacA4 gene was investigated by PCR. Integrase positive strains were analyzed for presence of resistance gene cassettes by sequencing. All the isolates were identified as MRSA by DDM and confirmed by PCR. All isolates were re sistant to ampicillin and cefoxitin. For Aminoglycosides, high resistance was reported for streptomycin (60.7%), intermediate for tobramycin (37.1 %) and gentamicin (36 %), and low for amikacin (15.9 %). All isolates were sensitive to piperacillin/tazobactam. Integron I was detected in 41 isolates (46.6 %), while integron II was detected in three isolates (3.4 %). Sequencing of integron I-cassette indicates high prevalence of addA gene variants mediating aminoglycoside resistance. The aacA4 gene was found in DNA of 31 isolates (35.22 %). This study revealed high prevalence MRSA. AacA4 gene and class I integron harboring aadA gene were predominant in MRSA isolates