Impact of animal saliva on the performance of rapid antigen tests for detection of SARS-CoV-2 (wildtype and variants B.1.1.7 and B.1.351)

Faculty Veterinary Medicine Year: 2021
Type of Publication: ZU Hosted Pages:
Authors:
Ibrahim Mohamed Sabet Sabet
Journal: Veterinary Microbiology Elsevier Volume:
Keywords : Impact , animal saliva , , performance , rapid antigen    
Abstract:
SARS-CoV-2 infects several animal species and SARS-CoV-2 variants of concern (VOCs) may even show (as in humans) enhanced inter- and intra-species transmission rates. We correlated sensitivity data of SARS-CoV-2 rapid antigen tests (RATs) to viral RNA genome equivalents analyzed by real-time reverse transcriptase- polymerase chain reaction (RT-PCR). Further, we checked their suitability for testing animals by assessing saliva and VOC effects. Viral loads up to 2 logs (RNA copy number) under the hypothetical SARS-CoV-2 infec-tivity threshold were detected by most analyzed RATs. However, while saliva from various animal species showed generally no adverse effects on the RATs’ analytical sensitivities, the detection of VOCs B.1.1.7 and B.1.351 was in some RATs inferior to non-VOC viruses
    
     
 
       

Author Related Publications

  • Ibrahim Mohamed Sabet Sabet, "Detection of SARS-CoV-2 variant B.1.1.7 in a cat in Germany", Elsevier, 2021 More
  • Ibrahim Mohamed Sabet Sabet, "Pathogenicity of Highly Pathogenic Avian Influenza Virus in naturally infected poultry in Egypt", PLOS ONE, 2015 More
  • Ibrahim Mohamed Sabet Sabet, "Abrogation of Marek’s disease virus replication using CRISPR/Cas9", Springer Nature, 2020 More
  • Ibrahim Mohamed Sabet Sabet, "Cas9-expressing chickens and pigs as resources for genome editing in livestock", National Academy of Sciences, 2021 More
  • Ibrahim Mohamed Sabet Sabet, "Marek’s disease virus: from novel viral interleukin-8 (vIL-8) splice variants to inhibition with CRISPR/Cas9", Freie Universität Berlin, 2020 More

Department Related Publications

  • Ahmed Abdelsamiea Hassan Aali, "عزل وتوصيف فيروس إلتهاب الشعب الهوائية المعدى فى الدواجن", لايوجد, 1900 More
  • Shimaa Mohamed Galal, "Cleavage site stability of Egyptian highly pathogenic avian influenza viruses in backyard chickens during 2009-2011", Journal of Microbiology, Immunology and Infection, 2013 More
  • Gamelat Kottb Farag Kotb, "Genetic Analysis of Infectious Bronchitis Virus Isolated from Egyptian Broiler Chicken Flocks between 2017 and 2018", Nexus academic, 2019 More
  • Fatma Mohamed Abdallah, "Genetic Analysis of Infectious Bronchitis Virus Isolated from Egyptian Broiler Chicken Flocks between 2017 and 2018", Nexus academic, 2019 More
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