Tanacetum sinaicum (Asteraceae) is a rare perennial herb growing wild in the mountains of Southern Sinai (Egypt). It is a medicinal and endangered plant. So, this work aimed to develop an in vitro propagation method for the conservation of this highly threatened plant. Sterile seedlings were used as a source of explants which were cultivated on Murashige and Skoog (M&S) media supplemented with different combinations of growth regulators for callus formation and induction of shoots and roots. M&S media with 1 mg/L kinetin (Kn) showed direct shoot induction. For root induction, excised shoots were transferred to M&S medium supplemented with 1 mg/L naphthalene acetic acid (NAA). Moreover, n-hexane extracts of wild and in vitro propagated plants were analyzed for their volatile constituents by gas chromatography-mass spectrometry (GC-MS) which resulted in the identification of 38 and 27 constituents, accounting for 87.75 and 75.51 % of their total composition; respectively.