Abstract Background: A healthy work environment for nurses is complex and involving several dimensions, includes several components and connection between the components. Definition of the practice environment was the organizational advantage of the work environments that assist or prevent professional nursing actual application. It constitutes great significant agents that can impact the quality and safety of care, such as autonomy, monitoring over the environment and creates something among physicians and nurses can create the actual application environment extra appropriate of the being developed as necessary for the health as well as provide something to best results for the patient, the nurses and the institution. Aim of this study: Assess the effect of nursing work environment and burnout on safety attitudes among nurses through investigating their effect on safety climate, teamwork climate, and job satisfaction. Subjects and Methods: Research design: A descriptive correlational study design was used. Setting: This study was conducted at Zagazig University Hospitals. Subjects: Stratified random sample of 384 nurses working at Zagazig University Hospitals. Tools of data collection: A questionnaire sheet was used for data collection, it includes four parts: 1) personal characteristics of the studied nurses. 2) the Korean version of the nursing work index–Revised scale (NWI-R).3) Maslach Burnout Inventory scale (MBI).4) Safety Attitudes Questionnaire Short Form (SAQ), 2006. Results: The results revealed that the highest mean percent scores were for safety climate, followed by nurses' work environment, while the lowest mean percent score was for job satisfaction. In addition, there were statistically significant positive correlations between nursing work environment, safety climate, teamwork climate, and job satisfaction. However, there were negative relations between burnout and safety climate, teamwork climate, and job satisfaction with significant relations only with safety climate, and job satisfaction. Conclusion: Nursing work environment was a good predictor of safety climate, teamwork climate, and job satisfaction. Moreover, burnout was a good predictor of safety climate, and job satisfaction, meanwhile, it was not predictor to teamwork climate. Recommendations: Hospitals' administrators and nursing managers should perform and preserve teaching as well as training programs aimed to make greater consciousness of healthy work environment and safety cases for nurses, moreover, involve in knowledge transfer activities that actively encourage the better application concerning the health, safety and being comfortable of nurses.