Biofloc technology (BFT), offers some potential advantages for improvements in water quality and growth of farmed fish reared in recirculation systems. One practical disadvantage of implementing a BFT system to culture fish is the need to add organic carbon to maintain a C:N ratio above 10. The present study evaluated the effect of using mannan oligosaccharides as a carbon source in a biofloc system with cultivated tilapia. MOS resulted in increased lactic acid bacterial count in the water and the intestinal tract, modulated immune response and resistance against Aeromonus hydrophila and improved the survival and growth of reared Nile tilapia (Oreochromis niloticus L.)