This trial was performed to study the effects of addition and/or substitution of various form of zinc sources (inorganic, organic and nano zinc) on performance, economic efficiency, blood metabolites and zinc concentration in tissue (breast muscle and liver) of broiler chickens. A total of 525 day old chicks were divided into seven groups with five replicates per group (75 chicks/group; 15 chicks/replicate). birds fed on 7 experimental diets; group 1: control basal diet without zinc supplement, group 2: 40 ppm inorganic Zn (ZnSO4), group 3: 40 ppm organic Zn methionine (Zn-Met), group 4: 40 ppm nano zinc oxide (nano ZnO), group 5: 20 ppm ZnSO4+20 ppm Zn-Met, group 6: 20 ppm ZnSO4+20 ppm nano ZnO, group 7: 20 ppm Zn-Met+20 ppm nano ZnO. This experiment was extended for 6 weeks. Individual body weights and feed residues were measured weekly. At the trial end, collect blood and tissue samples after slaughter five birds/group. The overall BW, BWG and FCR were significantly improved in the Zn supplemented groups. Return, net profit or partial and collective efficiency measures were significant different between all group. There was significant increase in concentrations of serum total protein, HDL-cholesterol and ALP in groups supplemented with Zn. Furthermore, there was significant increase in serum; breast and liver zinc concentration in experimental groups. Compared to inorganic-Zn form, supplementation and/or substitution with organic-Zn and/or nano-Zn form had a positive influence on the overall performance, Zn concentration in bird's serum, tissue, and increase return and net profit so; its addition and/or substitution will improve productive and economic efficiency.