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Comparative therapeutic effects of mesenchymal stem cells versus their conditioned media in alleviation of CCL4-induced liver fibrosis in rats : Histological and biochemical study
Faculty
Medicine
Year:
2019
Type of Publication:
ZU Hosted
Pages:
Authors:
Sarah Mohamed Mahmoud Mahmoud Abedelaal
Staff Zu Site
Abstract In Staff Site
Journal:
Journal of Medical Histology Journal of Medical Histology
Volume:
Keywords :
Comparative therapeutic effects , mesenchymal stem cells
Abstract:
Background: Viral hepatitis, drug abuse and metabolic diseases cause liver injury. Stem cell conditioned media (CM) alone can offer organ repair as compared to stem cell therapy. Objective: This study was designed to investigate role of bone marrow derived mesenchymal stem cells (BM-MSCs) CM in comparison to BM-MSCs in amelioration of liver injury in carbon tetrachloride (CCl4) induced liver fibrosis Materials and Methods: PKH26 -labeled MSCs were transplanted into one albino rats group with CCl4 induced liver fibrosis. MSCs-CM was injected in another group. Hematoxylin and eosin staining, Masson trichrome for collagen, Periodic Acid Schiff for glycogen, immunohistochemical and biochemical analysis were done to compare the morphological and functional liver regeneration among groups. The expression differences of CYP7A1, CYP27A1, glutathione reductase, glutathione peroxidase, albumin and interleukins were examined by real-time polymerase chain reaction (RT-PCR) and enzyme linked immunosorbent assay (ELISA). Results: MSCs and CM transplantation significantly increased antioxidant enzymes activity in injured liver. Also, gene expression levels attenuated by CCl4, were increased up to basal levels. CCl4 group showed different degrees of pathology with some severely affected lobules. MSCs group showed nearly normal organization apart from some dilated sinusoids and vacuolated cells. MSCs-CM injection revealed almost normalized liver histological and immunohistochemical picture. Conclusion: BM-MSCs conditioned medium is found to play a predominant therapeutic role in chronic liver fibrosis as compared to BM-MSCs.
Author Related Publications
Sarah Mohamed Mahmoud Mahmoud Abedelaal, "Umbilical cord blood-mesenchymal stem cells and carvedilol reduce doxorubicin- induced cardiotoxicity: Possible role of insulin-like growth factor-1", Biomedicine & Pharmacotherapy, 2018; (105) 1192–1204, 2018
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Sarah Mohamed Mahmoud Mahmoud Abedelaal, "Influence of Human Umbilical Cord Blood Mesenchymal Stem Cells on Cerebrolysin Amelioration of Neurological Deficit Following Cerebral Ischemia-Reperfusion Injury in Rats", Med. J. Cairo Univ., September 2018; 86 (5): 2543-2555, 2018
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Sarah Mohamed Mahmoud Mahmoud Abedelaal, "CEffect of Silver Nanoparticles Versus Titanium Dioxide Nanoparticles on the Lung of Adult Male Albino Rats: A histological and immunohistochemical study", Egyptian Journal of Histology, 2018
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Sarah Mohamed Mahmoud Mahmoud Abedelaal, "Effect of Silymarin versus Silymarin and Green Coffee Extract on Thioacetamide Induced Liver Injury in Adult Male Albino Rats (Histological and Immunohistochemical Study)", Journal of Medical Histology, 2018
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Sarah Mohamed Mahmoud Mahmoud Abedelaal, "Toxicity of Subacute Oral Zinc Oxide Nanoparticles on Testes and Prostate of Adult Albino Rats and Role of Recovery", Journal of Histology & Histopathology, 2019
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Department Related Publications
Sally Ahmed Sleim Elewa, "Effect of Orally Administered Zinc Oxide Nanoparticles on Albino Rat Thymus and Spleen", Wiley, 2017
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Asmaa Othman Sleim, "Effect of Orally Administered Zinc Oxide Nanoparticles on Albino Rat Thymus and Spleen", Wiley, 2017
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Zinab Abdallah Gohda, "Effect of Orally Administered Zinc Oxide Nanoparticles on Albino Rat Thymus and Spleen", Wiley, 2017
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Halle Alawi Hashim, "Dentate Gyrus in Aged Male Albino Rats (Histological and Tau- Immunohistochemical Study)", Egypt. J. Histol, 2010
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Nihad Fahmy Ahmed Ibrahim, "البحث الخامس تأثير الانسداد الجزئي لمخرج المثانة على التركيب النسيجي للمثانة البولية فى ذكر الجرذ البالغ ودور الإمداد ب-ال-أرجينين", The Egyptian Journal of Histology 2014; 37:492-505, 2014
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