This study was performed to evaluate the impact of natural red pigment (prodigiosin) extracted from local microorganism of Serratia marcescens as a natural colouring, antioxidant and antimicrobial agents in strawberry nectar. The local S. marcescens was identified by morphological, biochemical tests as well as sequencing a fragment of the 16S rDNA gene as new Egyptian strain namely S. marcescens A1A. The antioxidant scavenging of prodigiosin was examined by 2,2-diphenyl-1-picrylhydrazyl (DPPH). The antibacterial activity of prodigiosin was assayed against three bacterial indicators (B. cereus, Staph. aureus and E. coli) by measuring the inhibition zones using disc-diffusion assay on Muller Hinton Agar. The results revealed that the free radical scavenging activity was increased in the prepared solutions with increasing the concentration of prodigiosin. The prodigiosin of S. marcescens A1A has prohibited the growth of tested bacteria effectively. On the other hand, the fortification of strawberry nectar with prodigiosin pigment improved the antimicrobial, antioxidant activities, colour, taste, clarity, thickness and overall acceptability of resulted products.