Abstract Background: Preparation for marriage has become a popular intervention for many individuals planning to marry. Aim: To explore and compare the knowledge and attitude about premarital counseling among Hadhramout University Students. Subjects & Methods: A comparative descriptive design was used on 400 students (males & females) from Hadhramout University Colleges. The medical group is represented by College of Medicine & Health Sciences & College of Nursing and the non- medical group is represented by College of Arts & College of Administrative Sciences. Two types of tools were used for data collecting were (1) questionnaire was designed to cover data about students socio-demographic characteristics, and the students' knowledge regarding premarital counseling and care, (2)- A three point likert- like scale was used to assess the student's attitude toward pre-marital counseling. Results: The results of the present study revealed that only 5.5% & 22.5% of non-medical and medical groups of students respectively scored high level of knowledge about premarital counseling (P = 0.000). Also, the present study revealed that (68.5% & 59.5%) of medical and non-medical groups respectively expressed a positive attitude toward premarital counseling also, the present study revealed that the females had high level of knowledge score (23.8%) than males (9.3%) about premarital counseling (P = 0.000). There are statistically significant difference between medical and non-medical groups in knowledge and attitude toward premarital counseling. Therefore, it is recommended that; conducting health education services about premarital counseling in every college, booklet about premarital counseling should be available in every college in addition to every medical care services.