Detection of QTLs for Outcrossing-Related Traits in CSSL Population Derived from Primitive Japonica Accession Ludao in the Genetic Background of O. sativa spp. Japonica Restorer C-bao Using RSTEP-LRT Method

Faculty Agriculture Year: 2019
Type of Publication: ZU Hosted Pages:
Authors:
Journal: Agronomy MDPI Volume:
Keywords : Detection , QTLs , Outcrossing-Related Traits , CSSL Population    
Abstract:
The outcrossing traits in rice (Oryza sativa L.) affect the yield of hybrid seed production. Using a cytoplasmic male sterile (CMS) line with good outcrossing traits, such as short flag leaf length (FLL), narrow flag leaf width (FLW), wide flag leaf angle (FLA), and elongated panicle neck length (PNL), for hybrid rice seed production, it is possible to avoid the procedure of cutting flag leaves and make the supplementary pollination feasible by machine. In this study, a japonica restorer C-bao as the receptor parent and a primitive japonica accession Ludao as the donor parent were used to construct a chromosome segment substitution line (CSSL) population. The CSSL population was used to detect quantitative trait loci (QTLs) for the four outcrossing traits using a likelihood ratio test based on the stepwise regression (RSTEP-LRT) method. The CSSL population constructed consisted of 163 lines covering 90.7% of the donor genome. Among the seven QTLs detected in the CSSL population, four QTLs were detected in both years. qFLL-4 explained 6.70% of the two-year-averaged phenotypic variance, and the alleles from Ludao decreased FLL 5.1 cm. qFLA-1.1 and qFLA-1.2 explained 7.85% and 21.29% of the 2-year-averaged phenotypic variance respectively, and the alleles from Ludao increased FLA 17.38° and 31.50°. qPNL-8 explained 8.87% of the 2-year-averaged phenotypic variance, and the alleles from Ludao increased PNL 4.44 cm. These favorable alleles identified could be used to improve the outcrossing traits of parents for hybrid rice seed production in rice.
   
     
 
       

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  • Moaz Salah El Khedr Ali, "Mining of favorable alleles for seed reserve utilization efficiency in Oryza sativa by means of association mapping", Springer Nature, 2020 More
  • Moaz Salah El Khedr Ali, "Detection of QTLs for Outcrossing‐Related Traits in  CSSL Population Derived from Primitive Japonica  Accession Ludao in the Genetic Background of   O. sativa spp. Japonica Restorer C‐bao using  RSTEP‐LRT Method", MDPI, 2019 More
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  • Moaz Salah El Khedr Ali, "Favorable alleles mining for gelatinization temperature, gel consistency and amylose content in Oryza sativa by association mapping", Springer Nature, 2019 More

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