The aim of this research was to investigate the total phenolic compounds and anti-diabetic activity of both Carthamus tinctorius L. and its extract. HPLC analysis indicated the presence of some polyphenolic compounds which, responsible for their therapeutic potential. Treatment of aqueous extract significantly reduced the elevated serum levels of Glucose, Lipase, Aspartate amino transferase (AST) and Alanine amino transferase (ALT). 10% safflower flowers restored the reduced level of Uric acid, Creatinine and Urea nitrogen. A significant increase in HDL-c but decrease in LDL-c level was observed compared to induced control. In histopathological study aqueous extract showed minimal to mild multifocal and diffuse granular degeneration and mild to moderate lobular disarray compared to control group. Obesity is one of the most common metabolic diseases which is considered one of the global health problems. The present study aimed to screen anti-lipase effect of flowers of Carthamus tinctorius L.