Outcomes of Gallic Acid on Alternariol Induced Cyto-Morphic and Genotoxic In Vivo Changes in Parotid Gland: 4-HNE Incorporated

Faculty Medicine Year: 2019
Type of Publication: ZU Hosted Pages:
Authors:
Journal: Biomedicines MDPI Volume:
Keywords : Outcomes , Gallic Acid , Alternariol Induced Cyto-Morphic , Genotoxic    
Abstract:
Alternaria toxins are emerging mycotoxins that gained considerable interest with increasing evidence of their existence and toxicological properties. There is limited research and insufficient data about their in vivo hazardous effects. We designed this study to evaluate histopathological and genotoxic in vivo impacts of alternariol (AOH) on the parotid gland as well as to assess the competency of gallic acid (GA) in reversing these effects. Forty healthy adult male Wister rats were utilized and assigned equally on control, GA, alternariol and AOH+ gallic treated groups. Parotid gland samples from experimental groups were collected and then examined for histopathological, ultrastructural and immunohistochemical examination for 4-hydroxynonenal “4-HNE as lipid peroxidation marker” as well as Comet assay for DNA damage. Additionally, parotid tissue homogenates were tested for catalase “CAT”, superoxide dismutase “SOD” and malondialdehyde “MDA” levels. Our data proved that alternariol produced various histopathological and ultrastructural alterations of parotid acini as well as significant DNA damage, significant reduction of CAT and SOD enzymatic activity and significant boosting of 4-HNE immunohistochemical expression and MDA levels as compared to control group. On the other hand, gallic acid administration almost restored histological and ultrastructural parotid architecture, 4-HNE immune-expression and biochemical levels. Ultimately, we demonstrated alternariol-induced histopathological and genotoxic alterations on parotid gland as well as the competency of gallic acid in reversing these effects.
   
     
 
       

Author Related Publications

  • Mai Amin Mohamed Mahmoud Samak, "Could curcumin ameliorate titanium dioxide nanoparticles effect on the heart? A histopathological, immunohistochemical, and genotoxic study", Springer Berlin Heidelberg, 2019 More
  • Mai Amin Mohamed Mahmoud Samak, "Therapeutic potentials of mesenchymal stem cells on the renal cortexof experimentally induced hypertensive albino rats: Relevant role ofNrf2", Tissue and Cell, 2017 More
  • Mai Amin Mohamed Mahmoud Samak, "Fluoxetine pretreatment enhances neurogenic, angiogenic and immunomodulatory effects of MSCs on experimentally induced diabetic neuropathy", Cell and Tissue Research, 2018 More
  • Mai Amin Mohamed Mahmoud Samak, "Synergistic therapeutic effects of Vitis vinifera extract and Silymarin on experimentally induced cardiorenal injury: The pertinent role of Nrf2", elsevier., 2019 More
  • Mai Amin Mohamed Mahmoud Samak, "Therapeutic Prospects of Hydroxytyrosol on Experimentally Induced Diabetic Testicular Damage: Potential Interplay with AMPK Expression.", springer, 2019 More

Department Related Publications

  • Maha Zayed Mohammed Hassan, "THE EFFECT OF CHRONIC STRESS ON THE TESTIS OF ADULT ALBINO RATS AND THE POSSIBLE PROTECTIVE EFFECT OF ASTAXANTHIN SUPPLEMENTATION (HISTOLOGICAL, IMMUNOHISTOCHEMICAL AND BIOCHEMICAL STUDY)", journal of medical histology, 2018 More
  • Zinab Mansour Mohamed Salem, "the effect of experimental hypothyroidism on skin of adult male albino rats and therapeutic role of topical tri-iodothyronine ( histological and immunohistochemical study )", egyptian journal of histology, 2015 More
  • Nihad Fahmy Ahmed Ibrahim, "دور الكوإنزيم كيو10 في اصلاح تلف الخصية المحدث بالأكريلاميد في الجرذان البيضاء البيضاء الفطيمة (دراسة هستولوجية و هستوكيميائية مناعية)", The Egyptian Journal of Histology 2013; 36:164-174, 2013 More
  • Abeer Mohamed Azmi Zaky, "Effect of unilateral obstruction of vas deferens on ipsilateral epididymis of adult albino rats Abeer M. Azmy, Maha A. AbdAllah and Dalia A. Mohamed", Lippincott, 2012 More
  • Maha Amin Abdullah Ahmd, "Effect of unilateral hindlimb immobilization on ipsilateral soleus muscle morphology of adult male albino rats", Lippincott, 2013 More
Tweet