Identification and analysis of full-length transcripts involved in the biosynthesis of insecticidal lignan (+)-haedoxan A in Phryma leptostachya

Faculty Agriculture Year: 2019
Type of Publication: ZU Hosted Pages:
Authors:
Journal: Industrial crops and products Elsevier Volume:
Keywords : Identification , analysis , full-length transcripts involved , , biosynthesis    
Abstract:
Elucidating the biosynthetic pathway of bioactive ingredients will provide an excellent opportunity for their sustainable supply. Phryma leptostachya, a traditional insecticidal plant, is rich in lignans, among which (+)-haedoxan A (HA) is the major insecticidal ingredient, but its biosynthetic pathway remains unclear. Bioassay results showed that HA exhibited remarkably high insecticidal activity against Mythimna separata with an LC50 value of 26.67 mg/L, which was higher than that of 3 common botanical insecticides celangulin-V, rotenone, and pyrethrin, and approximate to that of 3 common chemical insecticides indoxacarb, chlorantraniliprole, and permethrin. The main accumulation space of HA and two other representative lignans (+)-phrymarolin I and II was localized in the roots of P. leptostachya by using high performance liquid chromatography (HPLC) and bioassay methods. A comparative transcriptome analysis was applied to identify and characterize the full-length transcripts of root, leaf, and stem tissues using a combination of next-generation sequencing (NGS) and single-molecule real-time (SMRT) sequencing, which provided a potential basis for further study the biosynthetic pathway of HA. This study is the first report on transcriptome analysis of P. leptostachya, and 13 isoforms probably involved in the HA biosynthesis were finally collected. In conclusion, these results contribute to further research into the biosynthetic pathway of HA and other bioactive lignans, and the transcriptome data provide a valuable resource for investigating this crucial insecticidal plant.
   
     
 
       

Author Related Publications

  • Ahmed Ali Ahmed Ali Ayoob, "FILED EVALUATION OF THIAMETHOXAM INSECTICIDE AND ITS MIXTURES WITH CERTAIN ADJUVANTS IN CONTROLLING LEGUMINOUS APHID, Aphis craccivora (KOCH) AND THEIR RESIDUES IN PLANTS AND SOIL", جامعة الزقازيق, 2015 More
  • Ahmed Ali Ahmed Ali Ayoob, "Reduction of soil contamination by cypermethrin residues using phytoremediation with Plantago major and some surfactants", Springier, 2019 More
  • Ahmed Ali Ahmed Ali Ayoob, "Insecticidal Activity of Four Lignans Isolated from Phryma leptostachya", MDPI, 2019 More
  • Ahmed Ali Ahmed Ali Ayoob, "Antifungal activity of pregnane glycosides isolated from Periploca sepium root barks against various phytopathogenic fungi", Elsevier, 2019 More
  • Ahmed Ali Ahmed Ali Ayoob, "Transcriptome analysis of Plantago major as a phytoremediator to identify some genes related to cypermethrin detoxification", Springer, 2020 More

Department Related Publications

  • Ibrahim Mohamed Kelany, "تجارب معملية على المستخلص المائى لبذور النيم", لايوجد, 1900 More
  • Mohammed Ahmed Ibrahim Yousef, "BIOLOGICAL CHARACTERISTICS AND PREDATION EFFICACY OF THE GREEN LACEWING, Chrysoperla carnea (STEPHENS) (NEUROPTERA: CHRYSOPIDAE) UNDER LABORATORY CONDITIONS", Zagazig J. Agric. Res., Vol. 42 No. (3) 2015, 2015 More
  • Elsayed Abdel Malek Elmadany Elsheikh, "Toxicity and biochemical effects of Neem Azal T/S, willow (Salix aegyptiaca L.) and Chasteberry (Vitex agnus-castus L.) on house fly, Musca domestica L. (Diptra : Muscidae)", Journal of Biopesticide, 2015 More
  • Mohammed Youssef Ibrahiem Hendawy, "Effect of processing on acetamiprid residues in eggplant fruits, Solanum melongena L.", academicjournals., 2013 More
  • Shadia Mostafa Taha Emara, "REPELLENCY AND FUMIGANT TOXICITY OF CLOVE AND SESAME OILS AGAINST AMERICAN COCKROACH: PERIPLANETA AMERICANA (L.)", أفريقيا, 2013 More
Tweet