Vanadium pentoxide (VP) is a well-defined industrial pollutant, known to adversely affect the reproductive functions of male. This work was done to study the protective activity of the quercetin against VP; we have studied reproductive alterations associated with VP by oxidative stress and deleterious changes on testicular function. Treatment with VP resulted in a reduction of serum total testosterone and luteinizing hormone (LH), with an elevation in the lactate dehydrogenase (LDH) levels. It led to a prominent spermatogenic arrest, decreased the motility percent of sperm and count, and also increased the sperm abnormality. The testicular acid phosphatase was elevated, and reduced glutathione (GSH) and catalase (CAT) levels were reduced, whereas malondialdehyde levels elevated. Additionally, VP reduced actin testicular cytoskeleton immunostaining in myoid cells. In response to concurrent treatment with quercetin and VP, serum hormones, LDH, and sperm quality were improved. Moreover, it reduced oxidative stress by reduction of malondialdehyde and elevation of GSH and CAT activity. Besides, increased actin cytoskeleton immunostaining in myoid cells.