Liquid Chromatographic and Potentiometric Methods for Determinations of Clopidogrel

Faculty Science Year: 2008
Type of Publication: ZU Hosted Pages:
Authors:
Journal: IEEE Access USA Volume:
Keywords : Liquid Chromatographic , Potentiometric Methods for Determinations , Clopidogrel    
Abstract:
Two different techniques are developed for the determination of clopidogrel in pharmaceutical preparations. HPLC method has been developed where chromatographic analysis is performed on Nova-Pak® C18 column (3.9 mm × 150 mm, 5 μm) with an ammonium formate buffer adjusted with formic acid to pH 4.0, acetonitrile (40:60, v/v) as mobile phase, and detection at 225 nm. Good linearity (0.9993, r), accuracy (≥ 99.20 %), and precision (≤ 0.6 RSD) were obtained. Potentiometric measurments are based on tetrakis (p-chlorophenyl) borate-clopidogrel ion-pair as an electroactive material incorporating a plasticized PVC membrane with o-nitrophenyl octyl ether or dioctyl phthalate. The sensor is conditioned for at least two days in 0.1 M drug solution before use. It exhibits fast and stable Nernstian response for clopidogrel over the concentration range of 1.0 × 10-5 ~ 1.0 × 10-2 M and pH range of 1.5 - 4.0. Results with an average recovery of 100.6% and a mean standard deviation of 0.86% of the nominal were obtained. The sensor shows reasonable selectivity towards clopidogrel hydrogen sulphate in presence of many cations. No significant interferences are caused by drug excipients and diluents
   
     
 
       

Author Related Publications

  • Mohamed Alaa Addeen Fathy Mohamed, "Lead(II) Potentiometric Sensor Based on1,4,8,11-Tetrathiacyclotetradecane Neutral Carrier and LipophilicAdditives", USA, 2008 More
  • Mohamed Alaa Addeen Fathy Mohamed, "Novel Sensors for Batch and Flow Injection Analysis of Histamine Based on Crown Ethers", USA, 2012 More
  • Mohamed Alaa Addeen Fathy Mohamed, "Poly (vinyl chloride) matrix membrane sensors for the quantification of olopatadine and oxeladine in pharmaceutical preparations and human plasma", USA, 2019 More
  • Mohamed Alaa Addeen Fathy Mohamed, "Recognition and Quantification of Some Monoamines Neurotransmitters", USA, 2016 More
  • Mohamed Alaa Addeen Fathy Mohamed, "New Potentiometric and Spectrophotometric Methods for the Determination of Dextromethorphan in Pharmaceutical Preparations", Japan, 2014 More

Department Related Publications

  • Mohamed Gamal Helmy Mohamed Abdelwahed, "Electronic Properties of Organic Complexes. 2. Reactive Azodyes", Spain, 1993 More
  • Mohamed Gamal Helmy Mohamed Abdelwahed, "Electrical Behaviour of some Azodye Mixed Ligand with Gd3+ and Er3+. b", Peru, 1994 More
  • Atef Mohamed AbdelHamid Ali Hassn, "heterocyclization of thiouracil derivative: synthesis of thiazolopyrimidines, tetrazolopyrimidines and triazolopyrimidines of potential biological activity", elsevier, 2015 More
  • Mohammed Aref Awad Allah Abu Zyd, "Synergistic positive effects of nano barium silicate on the hydration rate and phase composition of alkali-activated slag", Elsevier, 2022 More
  • Ahmed Abdelmoniem Ahmed Amer, "Synthesis and characterization of some calcium aluminate phases from nano-size starting materials", 0366-3175/© 2020 SECV. Published by Elsevier Espana, ˜ S.L.U, 2020 More
Tweet